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GSSG/GSH Quantification Kit II
货号: G263

氧化型/还原型谷胱甘肽定量试剂盒
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200 tests1560现货

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谷胱甘肽 (-L-谷氨酰-L-半胱氨酰-甘氨酸) 是体内的一种三肽化合物,它参与抗氧化和药物代谢的过程,还是谷胱甘肽过氧化物酶、谷胱甘肽S-转移酶、巯基转移酶等的底物。谷胱甘肽通常以还原型状态(GSH) 存在,但是GSH在氧化应激的作用下会转化为氧化型状态 (GSSG)。因此GSH/GSSG的比值被认为是氧化应激研究的一个重要指标。
GSSG/GSH Quantification Kit含有GSH掩蔽剂,加入掩蔽剂可以除去样品溶液中的GSH。因此可以通过检测加入DTNB (5,5’-dithiobis (2-nitrobenzoic acid)) 后在412nm处的显色反应加上在谷胱甘肽还原酶作用下的循环体系选择性地对GSSG进行定量。GSH含量则可以通过总谷胱甘肽含量减去GSSG含量来得到。本试剂盒总谷胱甘肽和GSSG的检测限分别为0.5 mol/l-50 mol/l0.5 mol/l-25 mol/l
试剂盒内含::
- Enzyme Solution ⋯⋯⋯⋯⋯⋯⋯⋯ 50 l×1                          - Coenzyme ⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯ ×2
       - Buffer Solution ⋯⋯⋯⋯⋯⋯⋯⋯⋯ 60 ml×1                          - Substrate (DTNB) ⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯ ×4
       - Standard GSH ⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯ ×1                           - Standard GSSG ⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯ ×1
       - Masking Solution ⋯⋯⋯⋯⋯⋯⋯⋯ 1 ml×1


参考文献
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