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  • 分 子 式: C34H20O7S2
  • 分 子 量: 604.65
  • 外      观: 白色-浅黄白色粉末或固体
  • 别      名:
  • 纯      度: ≧90.0% (HPLC)
  • 储存条件: -20
  • 运输条件: 室温
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SSP4
货号: SB10
3',6'-Di(O-thiosalicyl)fluorescein
CAS号:
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    SSP4是一种新的选择性检测硫烷硫的荧光探针。SSP4自身没有荧光,但是当它和硫烷硫反应后
会释放出较强的绿色荧光,因此SSP4可以成为一种检测硫烷硫的高灵敏的荧光探针。

《特长》
1. 与硫烷硫反应后释放出较强的荧光(ex 482nm, em 515nm)
2. 与硫烷硫的反应选择性高(不会与硫化氢、谷胱甘肽、半胱氨酸反应)
3. 可使用于细胞内成像
 
《反应机理》

《生体内存在的硫烷硫含有分子》
*生体内存在的硫烷硫会通过氧化还原及转移而变化。
 
《通过与硫烷硫反应的SSP4荧光光谱变化》
*在10 μmol/l SSP4(PBS)加入硫烷硫供体Na2S3(Sodium trisulfide)(终浓度:100 μmol/l)
 
《SSP4的反应选择性》
*10 μmol/l SSP4(PBS)对各种活体(10 μmol/l)的反应性(Y轴:以空白为1时的荧光强度比)
 
《硫烷硫含有分子的细胞内成像》
左图:未处理CHO细胞
右图:使用硫烷硫供体Na2S3(Sodium trisulfide)处理过的CHO细胞
 
 
《注意事项》
SSP4由于加入去垢剂偶尔会提高背景荧光。
*在10 μmol/l SSP4(1 %去垢剂/PBS)使用荧光酶标仪检测515 nm(Ex: 482 nm)的荧光强度

如需要使用TritonX-100,请将浓度调整到0.1 %以下。
对于NP-40、Tween20,浓度为1 %也可以使用,不过通过稀释也可以抑制背景荧光的提高。
 
《参考文献》
1)   W. Chen, C. Liu, B. Peng, Y. Zhao, A. Pacheco, and M. Xian, “New fluorescent probe for sulfane sulfurs and the application in bioimaging”, Chem. Sci., 2013, 4, 2892.
2)   T. Ida, T. Sawa, H. Ihara. Y. Tsuchiya, Y. Watanabe, Y. Kumagai, M. Suematsu, H. Motohashi, S. Fujii, T, Matsunaga, M. Yamamoto, K. Ono, N. O. Devarie-Baez, M. Xian, J. M Fukuto, and T. Akaike, “Reactive cysteine persulfides and S-polythiolation regulate oxidative stress and redox signaling”, Proc Natl Acad Sci U S A., 2014, 111, 7606.
3)   E. Marutani, M. Sakaguchi, W. Chen, K. Sasakura, J. Liu, M. Xian, K. Hanaoka, T. Nagano, and F. Ichinose, "Cytoprotective effects of hydrogen sulfide-releasing N-methyl-D-aspartate receptor antagonists mediated by intracellular sulfane sulfur", Med. Chem. Commun., 2014, 5, 1577.
4)   M. Sakaguchi, E. Marutani, H-S. Shin, W. Chen, K. Hanaoka, M. Xian, and F. Ichinose, “Sodium Thiosulfate Attenuates Acute Lung Injury in Mice”, Anesthesiology, 2014, 121, 1248
5)   Y. Kimura, Y. Toyofuku, S. Koike, N. Shibuya, N. Nagahara, D. Lefer, Y. Ogasawara, and H. Kimura, "Identification of H2S3 and H2S produced by 3-mercaptopyruvate sulfurtransferase in the brain", Nature Scientific Reports., 2015, 5, 14774.
6)   E. DeLeon, Y. Gao, E. Huang, M. Arif, N. Arora, A. Divietro, S. Patel, K. Olson, "A case of mistaken identity: are reactive oxygen species actually reactive sulfide species?", Am J Physiol Regul Integr Comp Physiol, 2016, 310(7), R549.
7)   P. Yadav, M. Martinov, V. Vitvitsky, J. Seravalli, R. Wedmann, M. Filipovic, and R. Banerjee, "Biosynthesis and Reactivity of Cysteine Persulfides in Signaling.", J Am Chem Soc., 2016, 138(1), 289
8)   A. Moustafa and Y. Habara, "Reciprocal interaction among gasotransmitters in isolated pancreatic β-cells", Free Radic. Biol. Med., 2016, 11, 47.
9)   A. Moustafa and Y. Habara, "Crosstalk between polysulfide and nitric oxide in rat peritoneal mast cells", Am. J. Physiol. Cell Physiol., (in press)
10) E. R. DeLeon, Y. Gao, E. Huang, M. Arif, N. Arora, A. Divietro, S. Patel, and K. R. Olson, "A case of mistaken identity: are reactive oxygen species actually reactive sulfide species?", American Journal of Physiology: Regulatory, Integrative and Comparative Physiology., 2015, 310, (7), 549.
11) N. Takahashi, FY Wei, S. Watanabe, M. Hirayama, Y. Ohuchi, A. Fujimura, T. Kaitsuka, I. Ishii, T. Sawa, H. Nakayama, T. Akaike and K. Tomizawa, "Reactive sulfur species regulate tRNA methylthiolation and contribute to insulin secretion", Nucleic Acids Res., 2016, doi: 10.1093/nar/gkw745.